On the virome's hidden diversity: lessons from RHDV.
Ana M Lopes1,2,3,4, Joana Abrantes1,2,5
1CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Campus de Vairão, Universidade do Porto, Vairão, Portugal.
Mbio
|October 19, 2023
Summary
High-throughput sequencing rapidly identifies unknown microbes, aiding in understanding emerging infectious diseases. Metatranscriptomics analysis helps decipher viral evolution and predict pathogen emergence.
Area of Science:
- Microbiology
- Virology
- Genomics
Background:
- Emerging infectious diseases pose significant risks to human and animal health globally.
- Predicting the emergence of novel pathogens remains a complex scientific challenge.
- High-throughput sequencing technologies offer powerful tools for microbial discovery.
Purpose of the Study:
- To illustrate the utility of metatranscriptomics in studying viral evolution.
- To demonstrate the application of advanced sequencing in identifying microbial diversity.
- To explore methods for deciphering pathogen emergence using genomic data.
Main Methods:
- Utilizing metatranscriptomics to analyze complex biological samples.
- Applying high-throughput sequencing for comprehensive microbial identification.
- Analyzing sequence data to understand viral genetic dynamics.
Main Results:
- Rapid identification of diverse microbial communities, including viruses.
- Insights into viral genetic variation and evolutionary patterns.
- Demonstration of metatranscriptomics as a key tool for pathogen surveillance.
Conclusions:
- Metatranscriptomics provides crucial data for understanding viral evolution.
- Advanced sequencing technologies accelerate the discovery of unknown microbial diversity.
- This approach aids in addressing the challenge of emerging infectious diseases.
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