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Live-Imaging Centriole Amplification in Mouse Brain Multiciliated Cells.
Amélie-Rose Boudjema1, Adel Al Jord2, Anne-Iris Lemaître3
1Institut de Biologie de l'École Normale Supérieure (IBENS), CNRS, UMR 8197, INSERM, U1024, Paris Sciences et Lettres (PSL), Research University, Paris, France.
Methods in Molecular Biology (Clifton, N.J.)
|October 19, 2023
Summary
Multiciliated cells (MCC) amplify hundreds of basal bodies to form motile cilia, essential for fluid movement. Our protocol enables live imaging of this critical centriole amplification process, aiding research into related human diseases.
Area of Science:
- Cell Biology
- Developmental Biology
- Human Pathologies
Background:
- Multiciliated cells (MCC) possess numerous motile cilia crucial for physiological fluid propulsion in various tissues.
- Motile cilia originate from basal bodies, which are derived from centrioles; MCC uniquely amplify centrioles for massive cilia formation.
- Defects in centriole amplification cause 'reduced generation of motile cilia' (RGMC), leading to hydrocephaly, lung failure, and subfertility.
Purpose of the Study:
- To present an optimized live-imaging protocol for observing centriole amplification dynamics in MCC.
- To highlight the utility of mouse brain MCC as a model system for studying centriole amplification.
- To provide practical guidance for monitoring this complex organelle reorganization.
Main Methods:
- Development and optimization of a live-imaging protocol.
- Utilizing mouse brain MCC as the model system.
- Spatially and temporally resolved monitoring techniques.
Main Results:
- Successful live imaging of centriole amplification dynamics in MCC.
- Demonstration of mouse brain MCC as a suitable model for this process.
- Establishment of methods for detailed observation of organelle reorganization.
Conclusions:
- The developed protocol facilitates unprecedented insights into centriole amplification.
- This research provides a valuable tool for understanding RGMC and related pathologies.
- The study enables further investigation into the mechanisms of massive organelle biogenesis.
