Related Experiment Video
Updated: Jul 12, 2025
10:38
Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
Published on: October 8, 2013
37.1K
Primary Human Nasal Epithelial Cell Culture
Mystica Terrance1, Tarini Gunawardena1, Hong Ouyang1
1The Hospital for Sick Children, Toronto, ON, Canada.
Methods in Molecular Biology (Clifton, N.J.)
|October 19, 2023
Abstract:
Primary human nasal epithelial cell (HNEC) cultures serve as an excellent model in studying physiology, disease, and therapy related to the respiratory tract. The current chapter details protocols from sample collection to culturing HNEC under air-liquid interface (ALI) conditions to generate well-differentiated, functional airway cultures including displaying beating motile cilia.
