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Related Experiment Video

Updated: Jul 12, 2025

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device
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Cortical spheroid on perfusable microvascular network in a microfluidic device.

Teal Russell1, Qassim Dirar1, Yan Li2

  • 1Fostering Innovation Through Biosystems for Enhanced Scientific Technologies (FIT BEST) Laboratory, Department of Chemical, Biological, and Bio Engineering, College of Engineering, North Carolina A&T State University, Greensboro, NC, United States of America.

Plos One
|October 19, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed a microfluidic platform to create vascularized human brain spheroids. This 3D model mimics brain development and offers a new tool for disease modeling and drug screening.

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Last Updated: Jul 12, 2025

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Area of Science:

  • Neuroscience
  • Biotechnology
  • Bioengineering

Background:

  • Human induced pluripotent stem cell (hiPSC)-derived brain spheroids model human brain development.
  • Existing models lack perfusable microvascular networks essential for homeostasis.
  • Angiogenesis, regulated by angiogenic factors, can create 3D vascular networks.

Purpose of the Study:

  • To develop a microfluidic platform for creating vascularized 3D brain spheroids.
  • To investigate factors influencing angiogenic vascular network formation.
  • To demonstrate a novel in vitro model for brain research and drug discovery.

Main Methods:

  • Constructed a microfluidic device with a 3D cortical spheroid.
  • Induced angiogenic sprouting using concentration gradient-driven angiogenic factors.
  • Investigated the effects of PMA, endothelial cell orientation, ECM, and spheroid integration on vascularization.

Main Results:

  • Successfully created a perfusable microvascular network integrated with a cortical spheroid.
  • Identified critical factors for successful angiogenesis and vascular network formation.
  • Demonstrated proof of concept for a membrane-free, scalable in vitro platform.

Conclusions:

  • A microfluidic system can generate vascularized brain spheroids, improving in vitro modeling.
  • This platform offers a cost-effective, animal-testing alternative for studying brain diseases and screening drugs.
  • The developed model holds potential for high-throughput applications in neuroscience research.