Related Experiment Video
Updated: Jul 12, 2025
02:36
Author Spotlight: Effective Reuse of Polycarbonate Tubes for Extracellular Vesicle Isolation
Published on: March 8, 2024
1.0K
Transcriptional differences between vesicular hand eczema and atopic dermatitis
Fieke M Rosenberg1, René Wardenaar2, Angelique N Voorberg1
1Department of Dermatology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Contact Dermatitis
|October 19, 2023
Summary
Vesicular hand eczema (VHE) shares pathophysiology with atopic dermatitis (AD), but unique gene expression patterns, including interferon signaling and aberrant epidermal differentiation, distinguish VHE from AD.
Area of Science:
- Dermatology and Molecular Biology
- Transcriptomics and Gene Expression Analysis
Background:
- Vesicular hand eczema (VHE) shares significant transcriptome overlap with atopic dermatitis (AD).
- Key differentially expressed genes (DEGs) distinguishing VHE from AD remain unidentified.
Purpose of the Study:
- To identify unique transcriptional signatures differentiating VHE from AD.
- To elucidate specific gene expression patterns in VHE compared to AD.
Main Methods:
- Re-analysis of RNA sequencing data from lesional palmar VHE epidermal biopsies.
- Comparative DEG analysis with lesional skin biopsies from AD patients.
- Identification of shared, unique, and oppositely regulated DEGs between VHE and AD.
- Gene set enrichment and Chi-squared analyses to compare disease-specific transcriptomic profiles.
Main Results:
- A total of 3028 DEGs in VHE and 5391 DEGs in AD were identified.
- 1516 DEGs were shared, while 1512 DEGs were unique to VHE.
- Interferon signaling and necroptosis pathways were significantly upregulated in VHE.
- Downregulated genes in VHE, such as DNASE1L2 and keratin genes, suggest aberrant epidermal differentiation.
Conclusions:
- VHE and AD share a common underlying pathophysiology.
- Distinct transcriptional profiles, particularly in interferon signaling and epidermal differentiation, differentiate VHE from AD.
Related Concept Videos
02:32Position-effect Variegation
6.3K
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
6.3K
01:33Pleiotropy
40.5K
Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
40.5K
01:15Reticular Dermis
2.6K
The papillary and reticular dermis are the two layers of the dermis. They are made of connective tissue with fibers of collagen extending from one to the other, making the border between the two somewhat indistinct. The dermal papillae extending into the epidermis belong to the papillary layer, whereas the dense collagen fiber bundles below belong to the reticular layer.
Reticular Layer
Underlying the papillary layer is the much thicker reticular layer, composed of dense, irregular connective...
Reticular Layer
Underlying the papillary layer is the much thicker reticular layer, composed of dense, irregular connective...
2.6K
01:30General Transcription Factors
5.3K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.3K
01:12Renewal of Skin Epidermal Stem Cells
2.5K
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
2.5K