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Murine Fecal Isolation and Microbiota Transplantation
Published on: May 26, 2023
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Washed microbiota transplantation improves renal function in patients with renal dysfunction: a retrospective cohort
Hao-Jie Zhong1,2,3, Xinqiang Xie2, Wen-Jia Chen1
1Department of Gastroenterology, Research Center for Engineering Techniques of Microbiota-Targeted Therapies of Guangdong Province, The First Affiliated Hospital of Guangdong Pharmaceutical University, Nonglinxia Road 19, Guangzhou, 510000, China.
Journal of Translational Medicine
|October 20, 2023
Summary
Washed microbiota transplantation (WMT) improved kidney function in patients with renal dysfunction by altering gut bacteria and increasing toxic metabolite excretion. This modified fecal microbiota transplantation method is safe and effective for chronic kidney disease treatment.
Area of Science:
- Microbiology
- Nephrology
- Gastroenterology
Background:
- Gut microbiota alterations are characteristic of chronic kidney disease (CKD).
- Targeting the gut microbiota offers a promising therapeutic strategy for CKD.
- Washed microbiota transplantation (WMT) is a modified fecal microbiota transplantation (FMT) approach.
Purpose of the Study:
- To assess the efficacy and safety of WMT in patients with renal dysfunction.
- To evaluate WMT's impact on renal parameters and gut microbiota composition.
- To analyze changes in urinary metabolites post-WMT.
Main Methods:
- Comparative analysis of gut microbiota in renal dysfunction patients versus healthy controls.
- Evaluation of WMT's effect on renal function markers (serum creatinine, eGFR, BUN).
- Analysis of gut microbiota and urinary metabolite profiles before and after WMT.
Main Results:
- Significant microbial community structure differences observed between patient and control groups.
- WMT significantly improved renal parameters (serum creatinine, eGFR, BUN) in patients.
- WMT increased gut microbiota diversity (Shannon index) and probiotic abundance, with a low adverse event rate (2.91%).
- Post-WMT urine showed increased levels of toxic metabolites: hippuric acid, cinnamoylglycine, and indole.
Conclusions:
- WMT is a safe and effective intervention for improving renal function in patients with renal dysfunction.
- WMT modulates the gut microbiota towards a healthier profile.
- WMT enhances the excretion of toxic metabolites, contributing to improved renal outcomes.
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