Three steps down: Metabolic depression in winter-acclimatized crucian carp (Carassius carassius L.)

Acclimatization of certain ectothermic vertebrates to winter conditions is associated with reduced energy consumption (winter dormancy). Principally, this may be achieved by reducing movement activity, depression of basal cellular functions, or by switching from aerobic to anaerobic energy production to sustain low energy consumption during anoxia. Therefore, we determined standard (SMR), routine (RMR) and anoxic (AMR) metabolic rates in summer- (SumA; 20 °C) and winter-acclimatized (WinA; 2 °C) crucian carp (Carassius carassius), an anoxia-tolerant teleost fish. At 20 °C, RMR was 39% lower in WinA than SumA fish (p < 0.05), indicating reduced movements in winter. SMR, measured for the first time in crucian carp, was 45% lower in WinA than SumA fish at 10 °C (p < 0.05), indicating significant reduction of energy consumption in vital cellular processes. At 2 °C, AMR - measured from ethanol production- was 78% and 97% of the SMR and RMR levels at the same temperature, respectively. The current study revealed that the winter dormancy in anoxia-tolerant crucian carp is achieved in 3 different steps: (1) by active reduction of SMR, possibly in anticipation of seasonal anoxia, (2) acute Arrhenius Q₁₀ effect that slows SMR as well as RMR, and (3) direct response to the absence of oxygen (AMR). Furthermore, the anoxic energy production was strongly dependent on body mass with scaling exponents of -0.335 and - 0.421 for WinA and SumA fish, respectively.