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Updated: Jul 12, 2025

Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2
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Blocking of doublecortin-like kinase 1-regulated SARS-CoV-2 replication cycle restores cell signaling network

Ram Babu Undi1,2, Nagib Ahsan3,4, Jason L Larabee5

  • 1Department of Radiation Oncology, University of Oklahoma Health Sciences Center , Oklahoma City, Oklahoma, USA.

Journal of Virology
|October 20, 2023
PubMed
Abstract

Insights

Targeting DCLK1 kinase offers a promising new strategy against severe COVID-19. Inhibiting DCLK1 effectively reduces SARS-CoV-2 replication and inflammation, aiding in treatment development.

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Severe COVID-19 and post-acute sequelae are significant health concerns, exacerbated by SARS-CoV-2 variants.
  • DCLK1 (Doublecortin-like kinase 1) is upregulated in COVID-19 lungs, promoting viral replication and hyperinflammation.

Purpose of the Study:

  • To investigate the role of DCLK1-regulated mechanisms in SARS-CoV-2 replication and pathogenic signaling.
  • To evaluate the therapeutic potential of DCLK1 kinase inhibition in COVID-19 treatment.

Main Methods:

  • Analysis of DCLK1 expression and its association with viral load and inflammatory markers in COVID-19 patients.
  • Pharmacological inhibition of DCLK1 kinase in SARS-CoV-2 infected cells.
  • Assessment of viral replication-transcription processes and cellular signaling pathways.

Main Results:

  • DCLK1 plays a critical role in driving SARS-CoV-2 replication-transcription and pathogenic signaling.
  • Pharmacological inhibition of DCLK1 kinase significantly impedes SARS-CoV-2 replication.
  • DCLK1 inhibition effectively counteracts virus-induced alterations in global cell signaling.

Conclusions:

  • DCLK1 kinase is a key driver of SARS-CoV-2 pathogenesis.
  • Targeting DCLK1 kinase represents a potential therapeutic strategy for managing severe COVID-19 and its sequelae.
Keywords:
COVID-19DCLK1DCLK1-IN-1SARS-CoV-2cell signalinghost-virus interactioninteractomepathologyreplication-transcription

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