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Detection, sequence patterns and function of unusual DNA structures.

J N Anderson

    Nucleic Acids Research
    |November 11, 1986
    PubMed
    Summary
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    Researchers discovered unusual DNA structures using gel electrophoresis. These bent DNA structures, found in yeast and adenovirus, may reside at the base of chromosomal DNA loops.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Biophysics

    Background:

    • DNA fragments exhibit unique migration patterns in electrophoretic procedures.
    • Oligomeric A tracts and periodic sequences can influence DNA structure and function.

    Purpose of the Study:

    • To investigate unusual DNA structures detected by electrophoretic separation.
    • To identify the characteristics and genomic locations of these anomalous DNA structures.

    Main Methods:

    • Electrophoretic separation of DNA fragments based on size (agarose gels) and shape (polyacrylamide gels).
    • Analysis of DNA sequences from yeast centromeres and specific loci, as well as adenovirus DNA.

    Main Results:

    • Yeast centromere fragments migrated faster than predicted, attributed to nonrandom A tract distribution.

    Related Experiment Videos

  • Anomalously slow migration of DNA fragments indicated bent DNA structures with periodic A-tracts.
  • Pronounced bent DNA sequences identified in yeast ARS1 and near adenovirus replication origins and enhancers.
  • Bent DNA sequences are associated with nuclear matrix attachment sites adjacent to enhancers.

    • Conclusions:

    • Bent DNA structures, characterized by specific sequence periodicities, are present in various genomic regions.
    • These bent DNA structures are located near crucial regulatory elements like replication origins and enhancers.
    • The findings suggest that bent DNA structures play a role in organizing DNA within chromosomal loops.