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Generation of Autologous Vascular Endothelial Cells for Patients with Peripheral Artery Disease
Bin Jiang1,2,3, Xinlong Wang4,5, Nancy Rivera-Bolanos4,5
1Center for Advanced Regenerative Engineering, Northwestern University, Evanston, IL, 60208, USA. bin.jiang@northwestern.edu.
Insights
Induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) show promise for treating peripheral artery disease (PAD). This approach offers a potential autologous cell source for vascular regeneration and limb salvage in PAD patients.
Area of Science:
- Regenerative Medicine
- Cardiovascular Biology
- Stem Cell Biology
Background:
- Peripheral artery disease (PAD) is a common condition increasing the risk of limb loss.
- Developing autologous cell sources for vascular regeneration is crucial for limb salvage in PAD patients.
Purpose of the Study:
- To establish patient-specific vascular endothelial cells (ECs) from peripheral blood for potential use in PAD regenerative therapy.
- To compare the efficacy of induced pluripotent stem cell (iPSC) and endothelial progenitor cell (EPC) approaches for generating autologous ECs.
Main Methods:
- Peripheral blood was collected from six PAD patients (age 50-80).
- ECs were derived using two methods: EPCs and iPSCs.
- Patient-derived iPSC-ECs were characterized for endothelial markers and functions.
- Inflammatory status (VCAM-1 expression) and response to resveratrol were assessed.
Main Results:
- The iPSC approach successfully generated patient-specific ECs in all PAD patients.
- The EPC approach failed to yield colony-forming ECs.
- Patient-derived iPSC-ECs expressed endothelial markers and functions but showed elevated VCAM-1.
- Resveratrol treatment demonstrated patient-specific responses in cell viability and VCAM-1 expression.
Conclusions:
- Patient-derived iPSC-ECs hold potential for autologous regenerative therapy in PAD.
- This approach offers a promising avenue for personalized treatment strategies for ischemic PAD.
- Further research is warranted to optimize iPSC-EC therapy for limb salvage.
Abstract:
Peripheral artery disease (PAD) is a prevalent cardiovascular disease with risks of limb loss. Our objective is to establish an autologous cell source for vascular regeneration to achieve limb salvage in PAD. Six PAD patients (age 50-80) were enrolled with their peripheral blood collected to derive vascular endothelial cells (ECs) with two different approaches: (1) endothelial progenitor cell (EPC) approach and (2) induced pluripotent stem cell (iPSC) approach. The iPSC approach successfully generated patient-specific ECs for all PAD patients, while the EPC approach did not yield any colony-forming ECs in any of the patients. The patient-derived iPSC-ECs expressed endothelial markers and exhibited endothelial functions. However, elevated inflammatory status with VCAM-1 expression was observed in the patient-derived cells. Pharmacological treatment with resveratrol resulted in patient-specific responses in cell viability and VCAM-1 expression. Our study demonstrates the potential of iPSC-ECs for autologous regenerative therapy in PAD, offering promise for personalized treatments for ischemic PAD.
