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Updated: Jul 12, 2025

Electrochemically and Bioelectrochemically Induced Ammonium Recovery
Published on: January 22, 2015
Improved regression model for anaerobic ammonium oxidation by repeated and prolonged batch assay under stressful
Kyungjin Cho1, Minkee Cho2, Shugyla Kaiyrlinova3
1Center for Water Cycle Research, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea; Division of Energy & Environment Technology, KIST School, University of Science and Technology (UST), Seoul 02792, Republic of Korea.
Abstract:
The aim of this study was to propose repeated and prolonged batch (RPB) assay as a promising specific anammox activity (SAA) methodology assessing the anammox activity under stressed salinity and pH conditions. Response surface analysis (RSA) was used as a regression tool to evaluate statistical significance. The feasibility of RPB was investigated at 0 to 15 g-NaCl/L of salinity and pH 6 to 8 with reflecting the results of preliminary SAA. As a result, conventional SAA was statistically insignificant. In addition, the RSA results obtained from repeated batch did not meet the statistical significance despite ten times iterative reaction. Interestingly, the RPB assay (i.e., applied both repeated and prolonged reaction) was effective to obtain the reliable results. Candidadus Brocadia and Candidadus Jettenia were functional anammox microbiome during RPB. Outcomes of this study suggest that RPB assay can be applied to accurately determine the anammox activity under various stressful conditions.
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