Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Single-trial conditioned place preference using intravenous morphine.

M T Bardo, J L Neisewander

    Pharmacology, Biochemistry, and Behavior
    |November 1, 1986
    PubMed
    Summary

    This study shows that a single morphine injection can create a conditioned place preference (CPP) in rats, highlighting the importance of immediate drug-environment pairing for reinforcing drug experiences.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Modeling escalation of drug intake to identify molecular targets for treating substance use disorders: A slippery slope upward.

    Neuroscience and biobehavioral reviews·2025
    Same author

    Effects of Social Isolation on Perineuronal Nets in the Amygdala Following a Reward Omission Task in Female Rats.

    Molecular neurobiology·2020
    Same author

    Effects of methamphetamine isomers on d-methamphetamine self-administration and food-maintained responding in male rats.

    Psychopharmacology·2019
    Same author

    Neuronal RNA-binding protein HuD regulates addiction-related gene expression and behavior.

    Genes, brain, and behavior·2017
    Same author

    In silico identification and in vivo validation of miR-495 as a novel regulator of motivation for cocaine that targets multiple addiction-related networks in the nucleus accumbens.

    Molecular psychiatry·2017
    Same author

    Adolescent methylphenidate treatment differentially alters adult impulsivity and hyperactivity in the Spontaneously Hypertensive Rat model of ADHD.

    Pharmacology, biochemistry, and behavior·2015

    Area of Science:

    • Neuroscience
    • Pharmacology
    • Behavioral Science

    Background:

    • Conditioned place preference (CPP) is a widely used behavioral paradigm to study drug reinforcement.
    • Understanding the critical parameters for establishing single-trial CPP is crucial for developing accurate animal models of addiction.

    Purpose of the Study:

    • To investigate the conditions necessary for establishing single-trial conditioned place preference (CPP) using intravenous morphine in rats.
    • To determine the influence of the timing of morphine administration relative to environmental cues on CPP acquisition.
    • To assess the role of opioid antagonists in blocking morphine-induced CPP.

    Main Methods:

    • Rats were exposed to a distinct white compartment paired with intravenous morphine administration (8 mg/kg) for 15 or 30 minutes.
    • Morphine administration was delayed to assess the impact of timing on CPP.
    • Naloxone (2 mg/kg, IV) was administered to block CPP acquisition.

    Main Results:

    • Single-trial CPP was successfully established when morphine was paired immediately with the white compartment for 15 or 30 minutes.
    • Delayed morphine administration (15 or 25 minutes after compartment exposure) prevented the development of single-trial CPP.
    • Naloxone administration blocked CPP acquisition, indicating an opioid-mediated mechanism, but did not alter baseline place preference.

    Conclusions:

    • Single-trial CPP acquisition is highly dependent on the immediate association between the drug experience and the environment.
    • This model provides a sensitive method to evaluate the reinforcing effects of the initial drug exposure.
    • The findings support the utility of the single-trial CPP model for studying the neurobiological basis of drug reinforcement and addiction.

    Related Experiment Videos