Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Connectivity biases generate a learning hierarchy in the <i>Drosophila</i> mushroom body.

bioRxiv : the preprint server for biology·2025

Same author

dArc1 controls sugar reward valuation in Drosophila melanogaster.

Current biology : CB·2025

Same author

<i>dArc1</i> controls sugar reward valuation in <i>Drosophila melanogaster</i>.

bioRxiv : the preprint server for biology·2024

Same author

Evolution of connectivity architecture in the Drosophila mushroom body.

Nature communications·2024

Same author

Evolution of connectivity architecture in the <i>Drosophila</i> mushroom body.

bioRxiv : the preprint server for biology·2023

Same author

Mushroom body input connections form independently of sensory activity in Drosophila melanogaster.

Current biology : CB·2022

Search research articles

Related Experiment Video

Updated: Jul 12, 2025

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

Published on: March 4, 2013

Mapping Kenyon cell inputs in Drosophila using dye electroporation.

Kaitlyn Elizabeth Ellis¹, Drue Marie Domagala¹, Sophie Jeanne Cecile Caron¹

¹School of Biological Sciences, University of Utah, Salt Lake City, UT 84112, USA.

|October 21, 2023

Summary

Researchers developed a new method to map connections to individual Kenyon cells in the Drosophila brain. This technique visualizes neuronal connections, enabling the analysis of brain circuit patterns.

Keywords:

Cell Biology Neuroscience

More Related Videos

Author Spotlight: Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos

Author Spotlight: Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos

Published on: April 14, 2023

Electrophysiological Recording in the Drosophila Embryo

Electrophysiological Recording in the Drosophila Embryo

Published on: May 21, 2009

Related Experiment Videos

Last Updated: Jul 12, 2025

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

Published on: March 4, 2013

Author Spotlight: Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos

Author Spotlight: Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos

Published on: April 14, 2023

Electrophysiological Recording in the Drosophila Embryo

Electrophysiological Recording in the Drosophila Embryo

Published on: May 21, 2009

Area of Science:

Neuroscience
Insect Neuroscience
Connectomics

Background:

Understanding neural circuits is crucial for deciphering brain function.
Kenyon cells are key components of the insect olfactory processing pathway.
Mapping neuronal connectivity provides insights into information processing.

Purpose of the Study:

To present a novel technique for charting the synaptic inputs of individual Kenyon cells in the Drosophila melanogaster brain.
To enable detailed analysis of neuronal connectivity patterns within the insect brain.

Main Methods:

Photo-labeling of single Kenyon cells per brain hemisphere to visualize dendritic terminals.
Dye-filled electrode backfilling of projection neurons connected to Kenyon cell dendrites.
Replication across hundreds of brains to construct a comprehensive connectivity matrix.

Main Results:

The developed technique allows for the visualization and charting of individual Kenyon cell inputs.
Statistical analysis of the connectivity matrix can identify patterns like random or biased connectivity.
This method provides a foundation for understanding the functional organization of olfactory circuits.

Conclusions:

The described protocol offers a robust method for mapping neuronal connections in Drosophila.
This technique facilitates the study of neural circuit organization and information flow.
Future research can leverage this method to explore complex brain functions.