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Published on: February 19, 2016
Rapid capture and quantification of food-borne spores based on the double-enhanced Fe3O4@PEI@Ag@PEI core-shell
Yaodi Zhu1, Jiaqi Tian2, Shijie Liu2
1College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, PR China; International Joint Laboratory of Meat Processing and Safety in Henan Province, Henan Agricultural University, Zhengzhou 450002, PR China; Henan Jiuyuquan Food Co., LTD., Postdoctoral Innovation Base, Henan Province, Yuanyang 453500, PR China.
Abstract:
To realize rapid capture and quantification of food-borne spores and prevent their potential harm, Fe3O4@PEI@Ag@PEI core-shell structure nanoparticles were combined with flower-like AgNPs for double enhancement and efficient capture of spores. The developed sensor showed excellent reproducibility and SERS enhancement factor (AEF) is 4.6 × 104. Orthogonal partial least-squares discrimination analysis and linear discriminant analysis accurately identified the three spores (Bacillus subtilis, Bacillus cereus, and Clostridium perfringens), and the qualitative identification accuracy of linear discriminant analysis was 100 %. Efficient enrichment of B. subtilis spores was realized within 5 min, with a detection limit of 3 cfu/mL. Spiked tests revealed that this sensor was effective in detecting spores in milk, orange juice, and water samples, with recovery ratio of 95.2-103.9 % and relative standard deviation of 3.1-7.7 %. Thus, the developed sensor was accurate and reliable, and could achieve rapid identification and quantitative detection of food-borne spores.
