Related Experiment Video
Updated: Jul 12, 2025

Detection of Extravascular Trypanosoma Parasites by Fine Needle Aspiration
Published on: August 7, 2019
Clear and simple detection of asbestos stained with two dyes for building materials collected from disaster and
Masaaki Tabata1, Ryota Haraguchi2, Mitsunori Yada1
1Department of Chemistry and Applied Chemistry, Faculty of Science and Engineering, Saga University, 1 Honjo-machi, Saga 840-8501, Japan.
Abstract:
Whenever houses are demolished or disasters occur, large quantities of building materials are discharged, which may contain asbestos. To prevent the damage caused by asbestos exposure, a rapid asbestos presence confirmation method is required at demolition sites or temporary disaster storage sites. It is difficult to confirm the presence of asbestos in waste building materials by simple observation. However, it can be confirmed by staining the materials with two dyes: methylene blue (MB) with positive charge, and erythrosine (RED-3) with negative charge, and using a stereomicroscope. The method was applied to samples collected from disaster and demolition sites. Asbestos was stained violet or reddish-purple, and the base material of the building materials remained blue. Using this method, even amateur workers can detect asbestos by means of an image in a different color than the building substrate. Furthermore, the present method detected asbestos more explicitly than the official method (JIS A 1482, 1483; detection limit is < 0.1%) recommended by the Japanese government. This cost-effective method is suitable for detecting asbestos at disaster and demolition sites. The mixture of MB and RED-3 formed nanoparticles of size 151 nm and surface charge of -34 mV that selectively stained asbestos. The staining mechanism was discussed.
More Related Videos
08:56In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage
Published on: January 11, 2012
15:01Confocal Laser Scanning Microscopy-Based Quantitative Analysis of Aspergillus fumigatus Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung
Published on: September 18, 2021
Related Concept Videos
Differential Staining Technique
Fixation and Sectioning
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
Karyotyping
Simple Staining Technique
Three-Dimensional Microscopy in Microbiology