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Updated: Jul 12, 2025

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
Published on: August 15, 2019
Microbial community acclimatization enhances bioplastics biodegradation and biogas production under thermophilic
Elisa Clagnan1, Mirko Cucina2, Raveena Vilas Sajgule3
1Gruppo Ricicla labs., Dipartimento di Scienze Agrarie e Ambientali - Produzione, Territorio, Agroenergia (DiSAA), Università degli studi di Milano, Via Celoria 2, 20133 Milano, Italy; Department for Sustainability, Biotechnologies and Agroindustry Division, ENEA, Italian National Agency for New Technologies, Energy and Sustainable Economic Development, Casaccia Research Center, Via Anguillarese 301, 00123 Rome, Italy.
Abstract:
This paper reports the results of a novel study of microbial acclimatization for bioplastics anaerobic degradation and conversion into biogas. Three sequential anaerobic digestion (AD) runs were carried out to favour microbial acclimatization to two different bioplastics, starch-based (SBS) and polyactic-acid (PLA). AD of SBS and PLA bioplastics was favoured by the acclimatization of the inoculum to the substrate after each run of AD. SBS conversion into biogas increased by 52 % (from 94 to 143 NL kgVS-1) and it was correlated with the enhanced growth of starch degrading bacteria such as Hydrogenispora, Halocella and Haloplasma. PLA anaerobic degradation increased by 97 % (from 395 to 779 NLbiogas kgVS-1) and it was related to the acclimatization of known PLA-degraders such as Tepidimicrobium, Methanothermobacter and Tepidanaerobacter. Microbial acclimatization appears a suitable and low-cost strategy to enhance bioplastics circularity by promoting their anaerobic biodegradation and conversion into biogas.
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