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OpenDEP: An Open-Source Platform for Dielectrophoresis Spectra Acquisition and Analysis.

Ioan Tivig1, Mihaela Georgeta Moisescu1, Tudor Savopol1

  • 1Biophysics and Cellular Biotechnology Department, Excellence Center for Research in Biophysics and Cellular Biotechnology, Faculty of Medicine, Carol Davila University of Medicine and Pharmacy, 8 Eroii Sanitari Blvd., Bucharest 050474, Romania.

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|October 23, 2023
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Summary

OpenDEP is a novel platform for dielectrophoretic (DEP) cell separation, using lab-on-a-chip technology and open-source software. It accurately determines cell electrical properties from DEP spectra, offering an accessible and versatile tool for cell analysis.

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Area of Science:

  • Biophysics
  • Electrical Engineering
  • Cell Biology

Background:

  • Dielectrophoretic (DEP) cell separation is a label-free technique using electric fields to manipulate cells based on electrical properties.
  • Analysis of DEP spectra is crucial for understanding cell electrical and morphological characteristics.

Purpose of the Study:

  • To introduce OpenDEP, a novel platform for acquiring and processing DEP spectra of suspended cells.
  • To enable the determination of DEP spectra and electric parameters using an integrated lab-on-a-chip and open-source software system.

Main Methods:

  • Development of a lab-on-a-chip device with indium tin oxide-coated slides to create an inhomogeneous alternating electric field.
  • Acquisition of microscopic images of cell distributions under varying electric field frequencies.
  • Utilizing custom Python software to convert images into DEP spectra and estimate cell electrical parameters (membrane and cytoplasm conductivities and permittivities).

Main Results:

  • The OpenDEP platform was validated against a commercial device (3DEP from DEPtech).
  • Successful estimation of membrane and cytoplasm conductivities and permittivities for DC3F and NIH 3T3 cell lines.
  • Demonstrated advantages including ease of manufacturing, robust statistical analysis, and compatibility with standard microscopes.

Conclusions:

  • OpenDEP provides an accessible, versatile, and statistically robust platform for label-free cell separation and electrical characterization.
  • The open-source nature and integration capabilities of OpenDEP facilitate broader adoption and further research in cell biophysics.