Green Synthesis of Nickel Oxide NPs Incorporating Carbon Dots for Antimicrobial Activities

A biosynthesis composite using the green synthesis of titled metal nanoparticles (nickel oxide nanoparticles, NiO NPs, and carbon dots, C-dots) was produced, characterized, and then applied for antimicrobial activities. NiO NPs were produced using the Croton macrostachyus (Bakkannisa) plant leaf extract and nickel nitrate (III) hexahydrate [Ni(NO₃)₂·2H₂O] as precursors, while C-dots were produced using citric acid and o-phenylenediamine (o-OPD). The distribution of the average particle size of the NiO NPs and NiO NPs@C-dots was 25.34 ± 0.12 and 24.95 ± 0.22 nm, respectively. The antimicrobial effects of the prepared materials were tested against the selected bacterial and fungal strains. Based on the outcomes of the bioassay, it was realized that both the bare and composite materials were effective against all bacterial strains. The composite's high surface area with strong inhibitive effective antimicrobial effects against bacterial and fungal strains were observed. Therefore, strong inhibitive effects of 21-24 and 22-26 mm were observed with NiO NPs and NiO NPs@C-dots, respectively.