Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrated SERS and Machine Learning Workflow for Nanoplastic Detection on a Plasmonic Membrane.

Analytical chemistry·2026
Same author

A practical approach to a single-chain variable fragment (scFv) biotinylating for immunohistochemical analysis.

MethodsX·2026
Same author

Curcumin Improves Redox Status and Insulin Sensitivity in the Periphery and Decreases Leptin Receptor Expression in the Arcuate Nucleus in Obese Diabetic Mice.

Molecular nutrition & food research·2026
Same author

Evidence of SARS-CoV-2 Exposure in Rodents from Rural Localities in the Yucatan Peninsula, Mexico.

Viruses·2026
Same author

Machine learning-augmented lateral flow assays for point-of-care infectious disease diagnostics.

Lab on a chip·2026
Same author

Expression of the VapD protein by <i>Helicobacter pylori</i> during intracellular infection.

Microbiology (Reading, England)·2026

Related Experiment Video

Updated: Jul 12, 2025

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

16.3K

Disposable Device for Bacterial Vaginosis Detection.

Mariana D Avila-Huerta1, Karina Leyva-Hidalgo1,2, Karen Cortés-Sarabia2

  • 1Centro de Investigaciones en Óptica, A. C., Loma del Bosque 115, Lomas del Campestre, León 37150, Guanajuato, Mexico.

ACS Measurement Science Au
|October 23, 2023
PubMed
Summary

A new disposable biosensor offers rapid, point-of-care diagnosis for bacterial vaginosis (BV) by detecting the biomarker Sialidase. This microfluidic paper-based device provides results in 20 minutes, improving accessibility for women's reproductive health.

More Related Videos

Author Spotlight: Revolutionizing Research on Vaginal Microbiome Interactions Using a Vaginal Chip
08:15

Author Spotlight: Revolutionizing Research on Vaginal Microbiome Interactions Using a Vaginal Chip

Published on: February 16, 2024

2.3K
Author Spotlight: Accelerating Diagnostic Accuracy with Direct Identification of Gram-Negatives from Blood Culture Bottles
09:07

Author Spotlight: Accelerating Diagnostic Accuracy with Direct Identification of Gram-Negatives from Blood Culture Bottles

Published on: May 24, 2024

939

Related Experiment Videos

Last Updated: Jul 12, 2025

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

16.3K
Author Spotlight: Revolutionizing Research on Vaginal Microbiome Interactions Using a Vaginal Chip
08:15

Author Spotlight: Revolutionizing Research on Vaginal Microbiome Interactions Using a Vaginal Chip

Published on: February 16, 2024

2.3K
Author Spotlight: Accelerating Diagnostic Accuracy with Direct Identification of Gram-Negatives from Blood Culture Bottles
09:07

Author Spotlight: Accelerating Diagnostic Accuracy with Direct Identification of Gram-Negatives from Blood Culture Bottles

Published on: May 24, 2024

939

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Point-of-Care Diagnostics

Background:

  • Bacterial vaginosis (BV) affects reproductive-age women, increasing susceptibility to other infections and complications like preterm labor.
  • Conventional BV diagnosis relies on microscopy, requiring trained personnel and laboratory facilities.
  • There is a growing demand for rapid, accessible point-of-care diagnostic tools for infectious diseases.

Purpose of the Study:

  • To develop a novel microfluidic paper-based analytical device for the rapid diagnosis of bacterial vaginosis (BV).
  • To detect Sialidase, a biomarker overexpressed in BV, using a sensitive immunosensing mechanism.
  • To provide an alternative to conventional diagnostic methods, enabling point-of-care testing.

Main Methods:

  • Utilized a microfluidic paper-based device with a graphene oxide-coated surface as a fluorescence quencher.
  • Employed an immunosensing mechanism to detect Sialidase, a BV biomarker.
  • Analyzed fluorescence changes (photoluminescence) of immunoprobes based on immunoreactions with Sialidase.

Main Results:

  • The developed paper-based test successfully detected Sialidase, a biomarker for BV.
  • The assay was completed within 20 minutes using a small sample volume (6 μL).
  • The device demonstrated effectiveness in discriminating between normal microbiota and BV in 14 clinical vaginal swab specimens.

Conclusions:

  • The novel microfluidic paper-based device offers a rapid and accessible method for BV diagnosis.
  • This disposable biosensor technology can facilitate point-of-care testing, reducing reliance on specialized labs.
  • The device represents a significant advancement in managing BV and preventing its associated reproductive health consequences.