Wool keratin/zeolitic imidazolate framework-8 composite shape memory sponge with synergistic hemostatic performance...

Area of Science:

• Biomaterials Science
• Nanotechnology
• Wound Healing Research

Background:

• Uncontrolled hemorrhage and wound infections are life-threatening, particularly with deep, non-compressible bleeding.
• Existing treatments face challenges in managing severe bleeding and preventing subsequent infections.
• Advanced wound care materials are needed to address these critical medical issues.

Purpose of the Study:

• To develop and evaluate a novel wool keratin/zeolitic imidazolate framework-8 (WK/ZIF-8) composite shape memory sponge.
• To assess the hemostatic and antibacterial capabilities of the WK/ZIF-8 composite sponge.
• To compare the performance of the WK/ZIF-8 composite sponge against commercial medical sponges in preclinical models.

Main Methods:

• Fabrication of a WK/ZIF-8 composite sponge by incorporating ZIF-8 nanoparticles into wool keratin.
• Evaluation of the composite sponge's mechanical properties, cell viability impact, and antibacterial efficacy.
• Assessment of hemostatic performance in Sprague-Dawley (SD) rat and rabbit hemorrhage models.
• In vitro and in vivo antibacterial testing to confirm anti-infection activity.

Main Results:

• The WK/ZIF-8 composite sponge demonstrated enhanced mechanical properties and reduced cytotoxicity compared to ZIF-8 alone.
• The composite sponge exhibited superior hemostatic performance in animal models, outperforming commercial sponges.
• Significant in vitro and in vivo antibacterial activity was observed, indicating effective anti-infection properties.
• The porous structure and shape recovery ability contributed to the sponge's hemostatic efficacy.

Conclusions:

• The WK/ZIF-8 composite sponge offers a promising solution for rapid hemorrhage control and infection prevention.
• Synergistic effects between wool keratin and ZIF-8 nanoparticles enhance both hemostasis and antibacterial action.
• This novel biomaterial holds potential for advancing wound management strategies and improving patient outcomes.