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Updated: Jul 12, 2025
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Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
Published on: June 9, 2017
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Long non-coding RNA generated from CDKN1A gene by alternative polyadenylation regulates p21 expression during DNA
Michael R Murphy1,2, Anthony Ramadei1,2, Ahmet Doymaz1
1Chemistry Department, Hunter College, The City University of New York, New York, NY 10021, USA.
Nucleic Acids Research
|October 23, 2023
Summary
A new lncRNA, SPUD, is induced by DNA damage, increasing p21 protein levels and promoting cell-cycle progression. This discovery reveals a novel post-transcriptional regulatory mechanism in the DNA damage response.
Area of Science:
- Molecular Biology
- Genetics
- Cell Biology
Background:
- Alternative Polyadenylation (APA) dynamically regulates gene expression.
- Widespread APA occurs in introns during the DNA damage response (DDR).
Purpose of the Study:
- To investigate DDR-activated APA events and their functional consequences.
- To identify and characterize a novel lncRNA involved in DDR.
Main Methods:
- Analysis of APA events in CDKN1A during DDR.
- Identification and characterization of the lncRNA SPUD.
- Investigation of SPUD's role in p21 regulation and cell-cycle control.
Main Results:
- A DDR-activated APA event in CDKN1A generates the lncRNA SPUD.
- SPUD is induced by DNA damage, partly via p53, and stabilized by HuR.
- SPUD enhances p21 protein levels, impacting cell-cycle and growth by modulating translational regulators.
Conclusions:
- SPUD represents a novel lncRNA-mediated post-transcriptional regulatory mechanism for p21 expression during DDR.
- This finding highlights the significant role of lncRNAs in DDR and cell-cycle control.
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