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SDBS induces multiple catalase conformations in a dose-dependent manner.

Ajamaluddin Malik1, Abdullah Alhomida1, Javed Masood Khan2

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International Journal of Biological Macromolecules
|October 23, 2023
PubMed
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Sodium dodecylbenzene sulphonate (SDBS) influences bovine liver catalase (BLC) fibrillation. At specific concentrations, SDBS induces amyloid-like structures in BLC, while higher concentrations restore its native alpha-helical structure.

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AggregationAmyloidBovine liver catalaseNeurodegenerativeSurfactant

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Area of Science:

  • Biochemistry
  • Protein Misfolding Diseases
  • Materials Science

Background:

  • Amyloid fibrils are associated with incurable diseases.
  • The mechanisms of small molecule-induced amyloid fibrillation are not fully understood.
  • Understanding these mechanisms is crucial for therapeutic development.

Purpose of the Study:

  • To investigate the effect of sodium dodecylbenzene sulphonate (SDBS) on bovine liver catalase (BLC) fibrillation.
  • To elucidate the conformational changes of BLC induced by SDBS.
  • To characterize the formation of amyloid-like structures.

Main Methods:

  • Turbidity measurements
  • Dynamic light scattering (RLS kinetics)
  • Intrinsic fluorescence spectroscopy
  • Thioflavin T (ThT) fluorescence assays
  • Far-UV circular dichroism (CD) spectroscopy
  • Transmission electron microscopy (TEM)

Main Results:

  • At pH 2.0, BLC transitions from an alpha-helical to a random coil structure.
  • Low SDBS concentrations (<0.1 mM) promote a native-like alpha-helical structure in randomly coiled BLC.
  • Intermediate SDBS concentrations (0.1–1.0 mM) induce BLC aggregation and amyloid-like structures.
  • High SDBS concentrations (>1.0 mM) restore the native-like alpha-helical structure to BLC at pH 2.0.

Conclusions:

  • SDBS concentration-dependently modulates BLC fibrillation and structure.
  • Amyloid-like structures are formed within a specific range of SDBS concentrations.
  • This study provides insights into surfactant-induced protein fibrillation relevant to disease mechanisms and therapeutic strategies.