Identification of microplastics extracted from field soils amended with municipal biosolids

Microplastic particles in arable soil are expected to impact the environment and potentially human health. The application of municipal biosolids (MBs) to agricultural land presents a further dilemma in that biosolids act as a fertilizer for crop growth, and a disposal pathway for wastewater treatment plants. They are also a direct path for emerging contaminants, such as microplastics to enter the terrestrial environment. Reliable methods are needed to identify and quantify microplastics, found in agricultural soils to determine how microplastics are being cycled in the terrestrial environment. In this study, we developed a method for extracting microplastics from soil, and characterized their composition and identity for particles sized 5 μm to 2 mm. Method development was initially completed using natural soils spiked with microplastics and MBs, followed by the analyses of soil sampled from an agricultural field where MBs were recently applied at a rate of 13 tons dw/ha. The procedures that used the spiked samples showed that microplastics can be reliably extracted from soil in a laboratory setting, and identified and semi-quantified by thermogravimetric analysis combined with Fourier-transform infrared spectroscopy (TGA-FTIR). However, when the same methods were applied to the soil samples collected from the agricultural field, reproducibility became a challenge, as the number and type of microplastics changed even within the same soils (i.e., collected the same day from the same exact location). The variation in reproducibility observed between laboratory and field samples underscores the significant heterogeneity present in the environment. This heterogeneity, in turn, affects the identification and quantity of microplastics detected, a phenomenon observed even when comparing different fields within a single treatment regimen.