Related Experiment Video

Updated: Jul 12, 2025

Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury
14:48

Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury

Published on: March 21, 2021

5.2K

Melatonin Alleviates Acute Respiratory Distress Syndrome by Inhibiting Alveolar Macrophage NLRP3 Inflammasomes

Li Zheng1, Wenyu Zhou1, Yutong Wu1

  • 1Department of Anesthesiology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.

Laboratory Investigation; a Journal of Technical Methods and Pathology
|October 23, 2023
PubMed

Insights

Melatonin (MEL) alleviates sepsis-induced acute respiratory distress syndrome (ARDS) by inhibiting the ROS/HIF-1α/GLUT1/NLRP3 pathway in macrophages via the MT1 receptor. This mechanism improves lung injury and survival in mice.

Area of Science:

  • Immunology
  • Respiratory Medicine
  • Molecular Biology

Background:

  • Sepsis-induced acute respiratory distress syndrome (ARDS) is a severe condition with no effective treatments.
  • Melatonin (MEL) shows potential for treating sepsis-induced ARDS, but its mechanism is not fully understood.

Purpose of the Study:

  • To elucidate the molecular mechanism by which melatonin alleviates sepsis-induced ARDS.
  • To investigate the role of the ROS/HIF-1α/GLUT1/NLRP3 pathway in melatonin's protective effects.

Main Methods:

  • Utilized a lipopolysaccharide (LPS)-induced mouse alveolar macrophage cell line (MH-S) model.
  • Investigated the effects of melatonin on NLRP3 inflammasome activation, glucose transporter 1 (GLUT1) expression, and hypoxia-inducible factor 1 (HIF-1α) levels.
  • Examined the role of reactive oxygen species (ROS) and melatonin receptor 1A (MT1) in melatonin's action.
  • Validated findings in an in vivo mouse model of LPS-induced lung injury.

Main Results:

  • Melatonin inhibited NLRP3 inflammasome activation in LPS-treated macrophages.
  • Melatonin downregulated GLUT1 by inhibiting HIF-1α, an effect modulated by ROS.
  • Melatonin's protective effect was dependent on the MT1 receptor.
  • In vivo, melatonin ameliorated LPS-induced lung injury and improved survival by downregulating the HIF-1α/GLUT1/NLRP3 pathway.

Conclusions:

  • Melatonin exerts protective effects against sepsis-induced ARDS by regulating the ROS/HIF-1α/GLUT1/NLRP3 pathway in alveolar macrophages.
  • The MT1 receptor mediates melatonin's action in this pathway.
  • These findings provide a mechanistic basis for melatonin as a potential therapeutic agent for sepsis-induced ARDS.
Keywords:
ARDSGLUT1HIF-1αNLRP3 inflammasomemelatonin

More Related Videos

Mouse Model of Oleic Acid-Induced Acute Respiratory Distress Syndrome04:45

Mouse Model of Oleic Acid-Induced Acute Respiratory Distress Syndrome

Published on: June 2, 2022

2.3K
Halogenated Agent Delivery in Porcine Model of Acute Respiratory Distress Syndrome via an Intensive Care Unit Type Device09:36

Halogenated Agent Delivery in Porcine Model of Acute Respiratory Distress Syndrome via an Intensive Care Unit Type Device

Published on: September 24, 2020

2.8K

Related Concept Videos

Acute Respiratory Failure-V01:29

Acute Respiratory Failure-V

The treatment for acute respiratory failure varies based on factors like the underlying cause, overall health, and severity. A collaborative healthcare team is essential for early detection, often through arterial blood gas analysis. Identifying the cause is the primary goal, with treatment strategies adjusted for ventilation/perfusion (V/Q) mismatch, shunting, or diffusion impairment.
Ensure that patients are monitored continuously for their response to therapy, including changes in...
143
Acute Respiratory Failure-II01:21

Acute Respiratory Failure-II

Type I Respiratory Failure, or hypoxemic respiratory failure, occurs when the partial pressure of oxygen (PaO2) in arterial blood falls below 60 mmHg while breathing room air without a corresponding increase in arterial carbon dioxide levels (PaCO2). This condition highlights a significant impairment in the lungs' capacity to oxygenate the blood.
The underlying physiological abnormalities that contribute to hypoxemic respiratory failure include:
249
Management of Insomnia01:19

Management of Insomnia

The sleep cycle, an integral part of human health, consists of several stages with distinct characteristics and functions. It begins with a transition from wakefulness to sleep, known as the light sleep phase, followed by the restorative deep sleep phase, essential for physical recovery and growth. The cycle concludes with the Rapid Eye Movement (REM) phase, characterized by high brain activity and vivid dreaming. Insomnia, a prevalent sleep disorder, involves difficulty falling asleep, staying...
260
Acute Respiratory Failure-IV01:23

Acute Respiratory Failure-IV

Respiratory failure can manifest suddenly or gradually, characterized by a rapid decline in PaO2 and a rapid rise in PaCO2. This situation indicates a severe respiratory problem that may quickly become a life-threatening emergency. One of the early signs of hypoxemic Acute Respiratory Failure (ARF) is a change in mental status due to the brain's sensitivity to oxygen levels and changes in acid-base balance. Symptoms such as restlessness, confusion, and agitation suggest inadequate oxygen...
160