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MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism

Julia Dluzewska1, Wojciech Dziegielewski1, Maja Szymanska-Lejman1

  • 1Laboratory of Genome Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznań, Poland.

Nature Communications
|October 23, 2023
PubMed

Insights

MSH2 regulates meiotic crossovers in Arabidopsis by opposing non-interfering crossovers at polymorphic sites. This finding reveals MSH2 as a key regulator of double-strand break repair and crossover patterns.

Area of Science:

  • Genetics
  • Molecular Biology
  • Plant Science

Background:

  • Meiotic crossovers are essential for accurate chromosome segregation.
  • Crossovers form via interfering or non-interfering pathways.
  • Sequence polymorphism between homologs influences crossover formation.

Purpose of the Study:

  • To elucidate the role of MSH2 in regulating both interfering and non-interfering meiotic crossovers.
  • To understand how MSH2 interacts with sequence polymorphism to shape the crossover landscape.

Main Methods:

  • Utilized Arabidopsis mutants with altered non-interfering crossover rates.
  • Combined these mutants with msh2 mutants.
  • Analyzed meiotic recombination patterns along chromosome arms.

Main Results:

  • MSH2 inhibits non-interfering crossovers at polymorphic loci, contrasting its effect on interfering crossovers.
  • Observed MSH2-independent crossover inhibition at highly polymorphic sites.
  • MSH2-dependent crossover increases occurred at boundaries of heterozygous/homozygous regions.

Conclusions:

  • MSH2 acts as a master regulator of meiotic double-strand break repair in Arabidopsis.
  • MSH2 exhibits antagonistic regulation of interfering and non-interfering crossovers.
  • MSH2 shapes the crossover landscape in response to interhomolog sequence polymorphism.

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