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Updated: Jul 12, 2025
05:37
Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
Published on: April 4, 2025
696
Detection and tracking of cytoplasmic G-quadruplexes in live cells
Lu-Si Rao1, Liang Hao1, Liu-Yi Liu1
1Key Laboratory of Bioinorganic and Synthetic Chemistry of Ministry of Education, School of Chemistry, IGCME, Sun Yat-Sen University, Guangzhou 510275, China. liuwenting@mail.sysu.edu.cn.
Chemical Communications (Cambridge, England)
|October 24, 2023
Abstract:
A TTPP probe was developed to distinguish G-quadruplexes (G4s) from other nucleic acid topologies through longer fluorescence lifetimes and higher quantum yields. In fluorescence lifetime imaging microscopy, TTPP enabled the visualization of cytoplasmic G4s in live cells, and showed the potential to detect cell apoptosis and ferroptosis by tracking cytoplasmic G4s.
