Motion Blur Microscopy.

Area of Science:

• Biomedical Engineering
• Cell Biology
• Microscopy

Background:

• Studying cellular adhesion in whole blood is vital for understanding biological functions and diseases.
• Conventional *in vitro* microscopy requires blood dilution, losing physiological complexity and crucial signaling cues.
• Existing methods fail to capture the full dynamics of cell interactions in native blood environments.

Approach:

• Motion blur microscopy (MBM) is a novel *in vitro* imaging technique using low light intensity and long integration times.
• MBM blurs flowing cells while highlighting adhered cells, enabling identification and tracking.
• Automated machine learning analysis processes large datasets of cell interactions in microfluidic channels under whole blood flow.

Key Points:

• MBM offers a low-cost, accessible alternative to intravital microscopy for studying adhesion dynamics.
• The method accurately quantifies cell size, eccentricity, adhesion duration, and velocity.
• Demonstrated applications include sickle red blood cells and CAR-T cell adhesion studies.

Conclusions:

• MBM facilitates reliable imaging and analysis of cell adhesion in whole blood flow.
• This technique generates rich datasets for understanding disease mechanisms in cancer, thrombosis, and inflammatory conditions.
• MBM is a versatile tool for both experimental research and theoretical modeling of cell adhesion.