[Assessment of irradiation uniformity using the results of a cytogenetic study of human peripheral blood lymphocytes]

A study was made of chromosome aberration during the I mitosis in PHA-stimulated lymphocytes from normal donors' peripheral blood following gamma-radiation in vitro in doses of 1 to 8 Gy. The dose-effect curves were plotted for different cytogenetic characteristics (the percentage of cells with dicentrics, dicentrics per 100 cells and aberrant cell). It is shown that during even or relatively even radiation, the doses calculated on the basis of the different cytological characteristics do not substantially differ and distribution of the dicentrics in the cells is governed by Poisson's law. During uneven radiation simulated by numeric experiments with formation of cell mixtures radiated in different doses, one could observe superdispersion of the cells with a different number of the dicentrics relative to Poisson's distribution, whereas the dose calculated according to the dicentrics (per 100 cells) was respectively higher and less than the doses calculated according to the number of the cells and dicentrics (per aberrant cell). A method for computerization of dicentric distribution in the cells to lymphocyte distribution according to the dose is described.