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Related Concept Videos

Proteomics01:33

Proteomics

7.4K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status
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Glial cell proteome using targeted quantitative methods for potential multi-diagnostic biomarkers.

Narae Kang1, Hyun Jeong Oh2,3, Ji Hye Hong1

  • 1New Drug Development Center, Heungdeok-gu, Chungbuk, Cheongju-si, 28160, Korea.

Clinical Proteomics
|October 24, 2023
PubMed
Summary

This study identified a 5-protein biomarker panel for glioblastoma, improving early diagnosis and treatment targeting. The panel shows high sensitivity and specificity, offering a promising tool for this aggressive brain cancer.

Keywords:
BiomarkerGlioblastomaMRMPrimary cellQuantitative proteomics

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Area of Science:

  • Neuro-oncology
  • Proteomics
  • Biomarker Discovery

Background:

  • Glioblastoma is a highly aggressive primary brain cancer with poor prognosis.
  • Current treatments like surgery, chemotherapy (temozolomide), and radiation have limited efficacy due to tumor resistance and recurrence.
  • There is a critical need for early diagnostic and molecularly targeted therapies for glioblastoma.

Purpose of the Study:

  • To identify novel glioblastoma-specific proteins for early diagnosis and targeted therapy.
  • To develop and validate a proteomic-based biomarker panel for glioblastoma detection.

Main Methods:

  • Comprehensive proteome analysis of primary glioblastoma cells and tissues.
  • Protein identification using Tandem Mass Tag (TMT) quantitative proteomics.
  • Validation of candidate biomarkers using Multiple Reaction Monitoring (MRM) assays with stable isotope standards (SIS) peptides.

Main Results:

  • Identified 7429 glioblastoma-specific proteins, with 476 quantified (228 upregulated, 248 downregulated).
  • A 5-protein panel (CCT3, PCMT1, TKT, TOMM34, UBA1) demonstrated significant differential expression (p ≤ 0.05, AUC ≥ 0.7).
  • The 5-marker panel achieved high sensitivity (0.80-0.90) and specificity (0.92-1.00) with improved AUC values (0.94-0.98) compared to single markers.

Conclusions:

  • A 5-protein biomarker panel shows potential for accurate glioblastoma detection in primary cells and tissues.
  • This panel offers improved diagnostic performance over single markers, aiding early diagnosis and prognosis.
  • Further validation in larger cohorts is warranted, but this panel serves as a baseline for novel glioblastoma biomarker discovery.