CRISPR screening in hematology research: from bulk to single-cell level

Area of Science:

• Molecular Biology
• Genetics
• Biotechnology

Background:

• CRISPR genome editing revolutionizes gene function studies.
• Genetic screens traditionally use bulk measurements.
• Single-cell technologies enable new screening approaches.

Purpose of the Study:

• To review bulk and single-cell CRISPR screening in research.
• To highlight the integration of CRISPR screening with single-cell multi-omics.
• To discuss the impact of these technologies on gene function analysis.

Main Methods:

• CRISPR screening for single or thousands of genes.
• Single-cell profiling combined with CRISPR screening.
• Multi-omics approaches including gene expression, chromatin accessibility, and protein levels.

Main Results:

• Single-cell CRISPR screening allows simultaneous monitoring of cell behavior and gene expression.
• Various Cas proteins expand the scope of CRISPR screens.
• Integration of single-cell multi-omics with CRISPR screening yields high-content data at single-cell resolution.

Conclusions:

• Single-cell CRISPR screening offers significant added value to research, particularly in hematology.
• Emerging technologies are rapidly overcoming current limitations in cell throughput and data density.
• This integrated approach opens new avenues for understanding genetic perturbations.