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Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens
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Predicting Blomia tropicalis allergens using a multiomics approach.

Jan Hubert1,2, Susanne Vrtala3, Bruno Sopko1

  • 1Crop Research Institute, Prague, Czechia.

Clinical and Translational Allergy
|October 25, 2023
PubMed
Summary

This study identifies numerous novel allergens in the domestic mite Blomia tropicalis using multiomics. Allergen gene expression doesn't always correlate with protein abundance in mite bodies.

Keywords:
IgEenzymegenomelabel-free proteomicsmitestranscriptome

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Area of Science:

  • Allergen research
  • Mite biology
  • Immunology

Background:

  • The domestic mite Blomia tropicalis is a significant allergen source in tropical regions.
  • Its allergome is poorly understood, with only 14 known allergen groups identified.
  • Previous studies suggest a larger number of allergens exist in B. tropicalis.

Purpose of the Study:

  • To comprehensively assess the allergome of Blomia tropicalis.
  • To identify novel allergens and determine the abundance of known and novel allergens.
  • To investigate the correlation between gene expression and protein abundance of allergens.

Main Methods:

  • Utilized a multiomics approach combining genomic and transcriptomic data.
  • Employed highly sensitive, label-free proteomics for protein quantification.
  • Performed sequence similarity analysis to predict novel allergens.

Main Results:

  • Confirmed 13 of 14 known allergen groups, identifying 16 potentially novel allergens.
  • B. tropicalis shares 27 known/deduced allergen groups with Dermatophagoides mites.
  • Identified Blo t 1, 5, 21, 15, and 18 as highly expressed at the transcript level, but Blo t 2, 10, 11, 20, 21, 3, 4, 6, 13, 14, and 36 showed higher protein abundance.

Conclusions:

  • An integrated omics method successfully identified and predicted numerous mite allergens.
  • Label-free proteomics revealed allergen protein abundance, differing from transcript levels.
  • Mite allergen gene expression levels do not strictly correlate with actual allergenic protein abundance.