Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

26
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
26
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

25
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
25
Genomics02:02

Genomics

36.4K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
36.4K
Next-generation Sequencing03:00

Next-generation Sequencing

89.0K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
89.0K
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

5.8K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
5.8K
Sanger Sequencing01:57

Sanger Sequencing

754.6K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
754.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The next frontier in forensic genetics: Single-cell genetic analysis and its expanding role.

Forensic science international. Genetics·2026
Same author

Challenges in mRNA-based time since deposition estimation: A multiplex MPS primer panel investigation.

Forensic science international. Genetics·2026
Same author

Probabilistic genotyping replicate analysis of FaSTR clustered single sperm aSTR haplotypes reconstitutes probative diploid DNA genotypes from complex semen mixtures.

Journal of forensic sciences·2025
Same author

mRNA profiling and donor association of mock casework samples: Results of a 3rd and 4th EDNAP collaborative exercise.

Forensic science international. Genetics·2025
Same author

Assessing Adventitious Matches of Non-Donors Related to True Contributors.

Biomolecules·2025
Same author

A collaborative study on the precision of the Markov chain Monte Carlo algorithms used for DNA profile interpretation.

Forensic science international. Genetics·2024

Related Experiment Video

Updated: Jul 12, 2025

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
11:49

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

15.8K

Single cell genomics applications in forensic science: Current state and future directions.

Kaitlin Huffman1, Jack Ballantyne2,3

  • 1Graduate Program in Chemistry, Department of Chemistry, University of Central Florida, PO Box 162366, Orlando, FL 32816-2366, USA.

Iscience
|October 25, 2023
PubMed
Summary

Subsampling individual cells (scDNA) from crime scene stains, instead of bulk DNA extraction, can reveal low-level DNA donors and resolve complex familial mixtures, enhancing forensic genomics accuracy.

Keywords:
BioinformaticsBiological sciencesGenomics

More Related Videos

Detection of Copy Number Alterations Using Single Cell Sequencing
09:45

Detection of Copy Number Alterations Using Single Cell Sequencing

Published on: February 17, 2017

11.7K
Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

20.4K

Related Experiment Videos

Last Updated: Jul 12, 2025

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
11:49

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

15.8K
Detection of Copy Number Alterations Using Single Cell Sequencing
09:45

Detection of Copy Number Alterations Using Single Cell Sequencing

Published on: February 17, 2017

11.7K
Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

20.4K

Area of Science:

  • Forensic Genomics
  • Molecular Biology
  • Genetics

Background:

  • Standard DNA mixture analysis uses bulk extraction, homogenizing all DNA.
  • This can obscure low-level contributors or familial relationships.
  • Single-source DNA (scDNA) profiling offers an alternative approach.

Purpose of the Study:

  • To review the history and applications of scDNA in forensic genomics.
  • To evaluate opportunities and challenges for scDNA implementation.
  • To highlight scDNA's potential for improved mixture analysis.

Main Methods:

  • Literature landscape review and commentary.
  • Analysis of subsampling techniques for cell isolation.
  • Evaluation of genetic analysis methods for scDNA profiles.

Main Results:

  • Subsampling individual cells can yield single-source DNA profiles.
  • This method enhances detection of low-level DNA donors.
  • scDNA analysis can resolve complex mixtures, including those with relatives.

Conclusions:

  • scDNA profiling presents a significant advancement in forensic genomics.
  • It offers solutions for challenging mixture interpretation and familial cases.
  • Further progress requires addressing current opportunities and impediments.