Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In Vitro Fertilization01:24

In Vitro Fertilization

305
In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...
305

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhancing Veterinary Education: Integrating Wildlife Medicine Into Undergraduate Training in Kenya.

Veterinary medicine international·2026
Same author

Sterility Test Conditions for Purified Sodium Hyaluronate Injection Evaluated via Membrane Filtration.

Biological & pharmaceutical bulletin·2026
Same author

Reconstitution of germ cell and gonadal development for in vitro gamete production.

The Journal of reproduction and development·2026
Same author

De-extinction of the Northern white rhinoceros (Ceratotherium simum cottoni).

The Journal of reproduction and development·2026
Same author

Changing Discourse on In Vitro Gametogenesis: Expectation, Scientific Reality, and the Ethics of Hype.

Monash bioethics review·2026
Same author

Vatican Revisits Ethics and Science of Xenotransplantation as Clinical Trials Advance.

The Linacre quarterly·2026

Related Experiment Video

Updated: Jul 12, 2025

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model
07:02

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model

Published on: May 16, 2019

12.7K

In vitro fertilization program in white rhinoceros.

Thomas Bernd Hildebrandt1,2, Susanne Holtze1, Silvia Colleoni3

  • 1Leibniz Institute for Zoo and Wildlife Research (IZW) in the Forschungsverbund Berlin eV, Reproduction Management, Alfred-Kowalke-Straße, Berlin, Germany.

Reproduction (Cambridge, England)
|October 25, 2023
PubMed
Summary

Assisted reproductive technologies are crucial for saving endangered rhinoceros species. This study successfully generated in vitro blastocysts for both Southern white rhinoceros (SWR) and, for the first time, Northern white rhinoceros (NWR), offering hope for conservation.

More Related Videos

Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases
10:59

Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases

Published on: November 18, 2013

18.3K
Bottlenose Dolphin Tursiops truncatus Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization
11:42

Bottlenose Dolphin Tursiops truncatus Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization

Published on: August 21, 2017

13.4K

Related Experiment Videos

Last Updated: Jul 12, 2025

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model
07:02

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model

Published on: May 16, 2019

12.7K
Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases
10:59

Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases

Published on: November 18, 2013

18.3K
Bottlenose Dolphin Tursiops truncatus Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization
11:42

Bottlenose Dolphin Tursiops truncatus Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization

Published on: August 21, 2017

13.4K

Area of Science:

  • Reproductive biology and conservation science.

Background:

  • Rhinoceros populations are declining, with several species critically endangered.
  • The Northern white rhinoceros (NWR) is extinct in the wild, and the Southern white rhinoceros (SWR) is Near Threatened.
  • New reproductive strategies are urgently needed to safeguard these species.

Purpose of the Study:

  • To assess the efficacy of an in vitro fertilization (IVF) program for Southern white rhinoceros (SWR) and Northern white rhinoceros (NWR).
  • To evaluate health effects, donor factors, and procedural elements of assisted reproduction in white rhinoceros.

Main Methods:

  • Over 8 years, 65 procedures were performed on 22 white rhinoceros females (20 SWR, 2 NWR).
  • Procedures included hormonal ovarian stimulation, ovum pick-up (OPU), in vitro oocyte maturation, fertilization, embryo culture, and blastocyst cryopreservation.
  • Efficiency was measured by blastocysts generated per OPU.

Main Results:

  • Generated 22 NWR, 19 SWR, and 10 SWR/NWR hybrid blastocysts.
  • Achieved an efficiency of 1.0 ± 1.3 blastocysts per OPU.
  • Collected data on health, donor, and procedural factors for future optimization.

Conclusions:

  • Successful in vitro blastocyst generation was achieved for both SWR and NWR.
  • This represents a significant advancement in assisted reproductive technologies for rhinoceros conservation.
  • The generated blastocysts offer potential for future breeding programs and species recovery.