Lysobacter arvi sp. nov. Isolated from Farmland Soil

Area of Science:

• Microbiology
• Bacteriology
• Genomics

Background:

• The genus Lysobacter comprises Gram-negative bacteria known for diverse metabolic capabilities.
• Soil environments are rich reservoirs of microbial diversity, including potentially novel bacterial species.

Purpose of the Study:

• To characterize a newly isolated bacterial strain from farmland soil.
• To determine the taxonomic position of the isolate within the Lysobacter genus.
• To propose a new species name based on comprehensive analyses.

Main Methods:

• Isolation and cultivation of bacteria from soil samples.
• 16S rRNA gene sequencing and phylogenetic analysis.
• Genome-based analyses including Average Nucleotide Identity (ANI) and digital DNA-DNA hybridization (dDDH).
• Analysis of chemotaxonomic features (fatty acids, quinones, polar lipids) and pigment production.

Main Results:

• The isolate, designated strain UC^T, is a Gram-negative, rod-shaped bacterium.
• 16S rRNA gene sequence analysis showed high similarity to Lysobacter soli DCY21^T (99.5%).
• Genomic analyses revealed ANI (87.7%) and dDDH (34.7%) values below the thresholds for species delineation with L. soli DCY21^T.
• Distinct phenotypic characteristics, including fatty acid profiles and xanthomonadin pigment production, were observed.
• Phylogenomic analysis supported the distinctness of strain UC^T.

Conclusions:

• Strain UC^T represents a novel species within the genus Lysobacter.
• The proposed name for the novel species is Lysobacter arvi.
• The type strain is UC^T (=KCTC 92613^T=JCM 23757^T=MTCC 12824^T).