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Short-Duration Hypothermia Induction in Rats using Models for Studies examining Clinical Relevance and Mechanisms
Published on: March 3, 2021
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INTRACOLON COOLING INCREASES SURVIVAL RATE IN THE RAT MODEL OF LETHAL HEMORRHAGE.
Awadhesh K Arya1, Kurt Hu2, Alice Chen3
1Department of Anesthesiology, University of Maryland School of Medicine, Baltimore, Maryland.
Shock (Augusta, Ga.)
|October 25, 2023
Summary
Transrectal intracolon (TRIC) cooling to 10°C significantly prolonged survival in a rat hemorrhagic shock model. This hypothermic TRIC intervention reversed hyperkalemia and prevented intestinal damage, enabling full resuscitation.
Area of Science:
- Physiology
- Medical Devices
- Trauma Research
Background:
- Hemorrhagic shock (HS) poses a critical threat, necessitating novel therapeutic strategies.
- Current treatments for HS have limitations in prolonging survival and preventing organ damage.
Purpose of the Study:
- To evaluate the efficacy of transrectal intracolon (TRIC) cooling in extending survival duration in a rat model of lethal hemorrhagic shock.
- To assess the impact of TRIC cooling on key physiological parameters and organ integrity post-hemorrhage.
Main Methods:
- Lethal HS was induced in rats by removing 47% of total blood volume.
- Intracolon temperatures were maintained at 37°C (TRIC37) or 10°C (TRIC10) using a TRIC device.
- Surface cooling (SC) was used as a comparative method; some TRIC10 rats underwent resuscitation (Res) at 90 minutes.
Main Results:
- TRIC10 significantly increased average survival time to 150.2 minutes, compared to 18.4 minutes for HS TRIC37 and 82 minutes for HS SC.
- TRIC10 prevented the post-HS rise in blood potassium levels and significantly reduced intestinal damage observed in other groups.
- All TRIC10 rats resuscitated at 90 minutes post-HS achieved full recovery.
Conclusions:
- TRIC10 cooling is a highly effective intervention for hemorrhagic shock, significantly prolonging survival.
- TRIC10 mitigates critical complications of HS, including hyperkalemia and intestinal injury.
- This hypothermic intracolon cooling strategy offers a promising approach for managing lethal hemorrhagic shock and enabling successful resuscitation.
