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Development of aptasensor for chlorpyrifos detection using paper-based screen-printed electrode.

Jayendra Kumar Himanshu1, G B V S Lakshmi2, Awadhesh Kumar Verma2

  • 1Special Centre for Nanoscience, Jawaharlal Nehru University (JNU), New Delhi, 110067, India; Department of Biotechnology, School of Life Sciences, Mahatma Gandhi Central University, Motihari, Bihar, 845401, India.

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|October 25, 2023
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Summary

A new electrochemical aptasensor using carbon quantum dots-graphite composite ink on screen-printed electrodes detects chlorpyrifos (CPF) with high sensitivity. This sensor shows excellent stability and applicability for detecting CPF in real food samples.

Keywords:
AptasensorChlorpyrifosElectrochemicalPotatoScreen-printed electrode

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Area of Science:

  • Electrochemistry
  • Nanomaterials Science
  • Analytical Chemistry

Background:

  • Chlorpyrifos (CPF) is a widely used organophosphate pesticide with significant environmental and health concerns.
  • Accurate and sensitive detection methods for CPF are crucial for food safety and environmental monitoring.
  • Existing detection methods often face limitations in sensitivity, selectivity, or practical application.

Purpose of the Study:

  • To develop a highly sensitive and selective electrochemical aptasensor for the detection of chlorpyrifos (CPF).
  • To utilize novel carbon quantum dots-graphite composite ink for fabricating screen-printed electrodes (SPEs).
  • To evaluate the performance and real-world applicability of the developed aptasensor.

Main Methods:

  • Fabrication of screen-printed electrodes (SPEs) using a carbon quantum dots-graphite composite ink.
  • Assembly of an electrochemical aptasensor by immobilizing specific aptamers targeting CPF.
  • Electrochemical characterization and performance evaluation using techniques like cyclic voltammetry and differential pulse voltammetry.
  • Testing the aptasensor's selectivity against other pesticides and its stability over time.
  • Validation of the aptasensor using spiked real food samples (potato extract).

Main Results:

  • The developed aptasensor exhibited a broad linear range for CPF detection from 1 pM to 500 nM.
  • A low limit of detection (LOD) of 0.834 pM was achieved, indicating high sensitivity.
  • The aptasensor demonstrated excellent selectivity against other common pesticides.
  • High stability was observed for the aptasensor electrode over five months of storage at 4°C.
  • The aptasensor showed good linearity and applicability when tested with spiked potato extract samples (R² = 0.981).

Conclusions:

  • The novel carbon quantum dots-graphite composite ink-based SPEs enable the development of a highly sensitive electrochemical aptasensor for CPF.
  • The aptasensor offers excellent sensitivity, selectivity, stability, and practical applicability for CPF detection in real food samples.
  • This technology holds promise for reliable monitoring of pesticide residues in the agricultural and food industries.