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Global repression by tailless during segmentation.

Lauro Hiroshi Pimentel Masuda1, Alan Utsuni Sabino2, John Reinitz3

  • 1Escola de Artes, Ciências e Humanidades da Universidade de São Paulo, São Paulo, Brazil.

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|October 25, 2023
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Summary
This summary is machine-generated.

The orphan nuclear receptor Tailless (Tll) represses key segmentation genes in Drosophila embryos. This study reveals Tll

Keywords:
DrosophilaEmbryoPatterningRepressionSegmentationTranscriptiontailless

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Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • The orphan nuclear receptor Tailless (Tll) has conserved roles in brain development.
  • Early tll expression in Drosophila segmentation is unusual, prompting investigation into its regulatory roles.

Purpose of the Study:

  • To investigate the regulation of tll on pair-rule stripes during Drosophila embryogenesis.
  • To elucidate the mechanisms by which Tll influences segmentation gene expression.

Main Methods:

  • Ectopic misexpression assays of tll.
  • Embryonic ChIP-chip analysis to identify Tll binding sites.
  • Mathematical modeling of gene regulation.
  • Genetic assays using mutant lines.

Main Results:

  • Ectopic Tll repressed pair-rule stripes (hairy, even-skipped, runt, fushi-tarazu) and gap genes (Kruppel, kni, giant).
  • ChIP-chip data confirmed Tll interactions with cis-regulatory modules (CRMs) of pair-rule and gap genes.
  • Mathematical modeling supported Tll acting as a repressor on even-skipped stripes.
  • Genetic assays showed synergistic repression of even-skipped stripe 5 by Tll and giant.

Conclusions:

  • Tll plays a direct role in repressing segmentation genes, particularly pair-rule stripes.
  • Tll acts combinatorially with other factors to restrict medial expression patterns during embryonic development.