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A simple, sensitive and colorimetric assay for Pseudomonas aeruginosa infection analysis

Jie Liu1, Dan Lu2, Junyuan Wang2

  • 1Obstetric Clinic, Chatu Hospital, the Fourth Hospital of Shijiazhuang City, Shijiazhuang City, Hebei Province, 050000, China.

Biotechniques
|October 26, 2023
PubMed

Insights

A new colorimetric assay offers a simple and sensitive method for detecting Pseudomonas aeruginosa (P. aeruginosa) infections. This rapid detection aids in the early diagnosis of postpartum skin infections.

Area of Science:

  • Microbiology
  • Biotechnology
  • Medical Diagnostics

Background:

  • Skin and soft tissue infections caused by Pseudomonas aeruginosa are prevalent in postpartum care.
  • Current detection methods for P. aeruginosa are often labor-intensive, complex, and costly.

Purpose of the Study:

  • To develop a simple, sensitive, and cost-effective colorimetric assay for P. aeruginosa detection.
  • To provide a tool for on-site testing and early diagnosis of postpartum infections.

Main Methods:

  • A ligation-initiated multiple-signal amplification method was employed.
  • The assay utilizes a colorimetric readout, displaying green for positive and colorless for negative samples.

Main Results:

  • The assay demonstrated a wide detection range.
  • A low limit of detection of 45 CFU/ml was achieved.
  • The method is simple, sensitive, and colorimetric.

Conclusions:

  • The developed assay is suitable for on-site testing of pathogenic bacteria.
  • This method can assist in the early diagnosis of postpartum care skin infections caused by P. aeruginosa.
Keywords:
G-quadruplexP. aeruginosaPseudomonas aeruginosacolorimetricpostpartum care infection

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