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Silencing Lnc-HES1-10 Inhibits Osteosarcoma Cells Proliferation, Invasive Ability, and Metastasis
Yingchun Zhu1, Chi Zhang, Caihua Zhang
1Department of Orthopedic Surgery, Ningbo First Hospital, Ningbo, China.
Background:
Long noncoding RNA (LncRNA) play a vital role in the development and pathophysiology of osteosarcoma (OS). However, the LncRNA activated by HES1-10 in OS has not been furthered investigated. This present study aims to show the possible function of Lnc-HES1-10 in OS.
Methods:
Cell proliferation in vitro were assessed by the MTT assay, whereas the migration and invasion abilities of OS cell lines were measured by wound-healing migration assay and transwell invasion assay, respectively. Quantitative reverse transcriptase polymerase chain reaction and western blot analysis was used to detected the expression level of HES1-10.
Results:
The present study demonstrated that the Lnc-HES1-10 is overexpressed in OS and associated with poor prognosis of patients. In addition, the results revealed that Lnc-HES1-10 is overexpressed in MG63 and 143B OS cell lines and promote proliferation on both cell lines in vitro. Furthermore, migration and invasion abilities of MG63 and 143B cells are suppressed after silencing Lnc-HES1-10.
Conclusion:
Our finding demonstrates that HES1-10 plays a crucial role in regulating OS growth and metastasis.
Insights
Long noncoding RNA HES1-10 (Lnc-HES1-10) is overexpressed in osteosarcoma (OS), promoting tumor growth and metastasis. Silencing Lnc-HES1-10 suppressed proliferation, migration, and invasion in OS cells, indicating its crucial role.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Long noncoding RNAs (LncRNAs) are implicated in osteosarcoma (OS) development and pathology.
- The specific role of LncRNAs activated by HES1-10 in OS remains underexplored.
Purpose of the Study:
- To investigate the function of Lnc-HES1-10 in osteosarcoma.
- To determine the impact of Lnc-HES1-10 on OS cell proliferation, migration, and invasion.
Main Methods:
- Cell proliferation assessed using MTT assay.
- Migration and invasion evaluated via wound-healing and Transwell assays.
- Quantitative reverse transcriptase polymerase chain reaction and Western blot used to determine HES1-10 expression levels.
Main Results:
- Lnc-HES1-10 was found to be overexpressed in OS tissues and associated with poor patient prognosis.
- Overexpression of Lnc-HES1-10 in MG63 and 143B OS cell lines promoted in vitro proliferation.
- Silencing Lnc-HES1-10 significantly suppressed the migration and invasion capabilities of MG63 and 143B cells.
Conclusions:
- Lnc-HES1-10 plays a critical role in regulating osteosarcoma cell growth and metastasis.
- Targeting Lnc-HES1-10 may represent a potential therapeutic strategy for osteosarcoma.
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