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Heterologous expression of fungal L-asparaginase: a systematic review
Kellen Cruvinel Rodrigues Andrade1, Joel Antonio Cordeiro de Abreu1, Marina Borges Guimarães1
1Laboratory of Natural Products, Health Science School, University of Brasília, Brasília, 70910-900, Brazil.
Abstract:
Aim: To review the available literature about heterologous expression of fungal L-asparaginase (L-ASNase). Materials & methods: A search was conducted across PubMed, Science Direct, Scopus and Web of Science databases; 4172 citations were identified and seven articles were selected. Results: The results showed that heterologous expression of fungal L-ASNase was performed mostly in bacterial expression systems, except for a study that expressed L-ASNase in a yeast system. Only three publications reported the purification and characterization of the enzyme. Conclusion: The information reported in this systematic review can contribute significantly to the recognition of the importance of biotechnological techniques for L-ASNase production.
