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Glycopeptide Capture for Cell Surface Proteomics
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Conformationally Restricted Glycopeptide Backbone Inhibits Gas-Phase H/D Scrambling between Glycan and Peptide

Christian Code1,2, Danwen Qiu1, Ilia A Solov'yov2,3,4,5

  • 1Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, 2100 Copenhagen, Denmark.

Journal of the American Chemical Society
|October 26, 2023
PubMed
Summary

Collision-induced dissociation (CID) of glycopeptides can reveal glycan deuterium content. However, hydrogen/deuterium (H/D) scrambling during fragmentation prevents accurate analysis in most glycoproteins, unlike in conformationally restricted molecules like vancomycin.

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