Dominant factors analyses and challenges of anaerobic digestion under cold environments

With the development of fermentation technology and the improvement of efficiency, anaerobic digestion (AD) has been playing an increasingly primary role in waste treatment and resource recovery. Temperature is undoubtedly the most important factor because it shapes microbial habitats, changes the composition of the microbial community structure, and even affects the expression of related functional genes. More than half of the biosphere is in a long-term or seasonal low-temperature environment (<20 °C), which makes psychrophilic AD have broad application prospects. Therefore, this review discusses the influencing factors and enhancement strategies of psychrophilic AD, which may provide a corresponding reference for future research on low-temperature fermentation. First, the occurrence of AD has been discussed. Then, the adaptation of microorganisms to the low-temperature environment was analyzed. Moreover, the challenges of psychrophilic AD have been reviewed. Meanwhile, the strategies for improving psychrophilic AD are presented. Further, from technology to application, the current situation of psychrophilic AD in pilot-scale tests is described. Finally, the economic and environmental feasibility of psychrophilic AD has been highlighted. In summary, psychrophilic AD is technically feasible, while economic analysis shows that the output benefits cannot fully cover the input costs, and the large-scale practical application of psychrophilic AD is still in its infancy. More research should focus on how to improve fermentation efficiency and reduce the investment cost of psychrophilic AD.