Related Experiment Video
Updated: Jul 12, 2025
Polysome Profiling in Leishmania, Human Cells and Mouse Testis
Published on: April 8, 2018
Preliminary Information of Iranian Lizard Leishmania Promastigote Transcriptome Sequencing by Next Generation
Farveh Ehya1, Sima Kalantari2,3, Mojgan Bandehpour4,5
1Department of Biotechnology, Tarbiat Modares University, Tehran, Iran.
Background:
A lizard Leishmania has been isolated from a lizard (Agama agilis) in Iran. Its genome sequence has not been determined, so far.
Methods:
The study was done at Shahid Beheshti University of Medical Sciences, Tehran, Iran in 2017-2023. Leishmania promastigotes were cultured in RPMI1640 culture medium and collected at logarithmic phase by centrigugation. Parasite RNA was extracted by the Qiagene standard kit and its quantity and quality was determined and sequenced by NGS method with Illumina PE machine at BGI Company (China).
Results:
The number of 8316 mRNA, 83 tRNA, 63 rRNA, 83 ncRNA, 5 snRNA, 1039 snoRNA, 36 region, and 3 repeat regions, 8343 CDS, 9597 Exon and 9292 Genes were identified in promastigote of Iranian lizard Leishmania.
Conclusion:
Genomic elements of Iranian lizards Leishmania (with unique characteristics) were determined and identified by NGS system.
