Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

494
Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
494
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

1.4K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.4K
Mass Spectrometers01:16

Mass Spectrometers

5.6K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
5.6K
Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

620
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
620
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

690
The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
690
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

1.0K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...
1.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reduction of the collective dynamics of neural populations with realistic forms of heterogeneity.

Physical review. E·2021
Same journal

How "Soft" Are Your Gas Mixtures? Effects of Modifier Gas Types on the Dissociation of Labile Ions in Differential Mobility Spectrometry.

Journal of the American Society for Mass Spectrometry·2026
Same journal

A Robotic Sample Handling Platform for Fully Automated Nanospray Desorption Electrospray Ionization Mass Spectrometry Imaging.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Direct Analysis in Real-Time Tandem Mass Spectrometry for Rapid Screening of Thirty-one Plant Growth Regulator Residues in <i>Rehmannia glutinosa</i>.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Characterization of Alkane Oxidation Products in a Corona-Discharge Reactor Using Ammonia-Doped Ion Mobility-Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Integration of a Modified Synchrotron Radiation Photoionization Time-of-Flight Mass Spectrometer with a Residual Gas Analyzer for Complementary Detection of Catalytic Products with Different Ionization Energies.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Screen for Tissue-Specific Markers of Drug-Induced Phospholipidosis Using Mass Spectrometry Imaging.

Journal of the American Society for Mass Spectrometry·2026
See all related articles

Related Experiment Video

Updated: Jul 12, 2025

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
08:43

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

Published on: May 11, 2017

12.4K

Modeling Known Sources of Mass Calibration Deviations in High Resolution ToF MS.

Boris Kozlov1, Sergey Kirillov2

  • 1Waters Corporation, Stamford Avenue, Altrincham Road, Wilmslow, Cheshire, U.K. SK9 4AX.

Journal of the American Society for Mass Spectrometry
|October 27, 2023
PubMed
Summary
This summary is machine-generated.

Accurate mass calibration in time-of-flight mass spectrometry requires accounting for distortions. Analytical modeling and numerical verification identified key factors affecting precise mass measurements.

More Related Videos

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

4.3K
Mass Cytometry: Protocol for Daily Tuning and Running Cell Samples on a CyTOF Mass Cytometer
10:59

Mass Cytometry: Protocol for Daily Tuning and Running Cell Samples on a CyTOF Mass Cytometer

Published on: November 2, 2012

22.9K

Related Experiment Videos

Last Updated: Jul 12, 2025

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
08:43

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

Published on: May 11, 2017

12.4K
Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

4.3K
Mass Cytometry: Protocol for Daily Tuning and Running Cell Samples on a CyTOF Mass Cytometer
10:59

Mass Cytometry: Protocol for Daily Tuning and Running Cell Samples on a CyTOF Mass Cytometer

Published on: November 2, 2012

22.9K

Area of Science:

  • Analytical Chemistry
  • Spectrometry

Background:

  • Modern time-of-flight mass spectrometers achieve parts-per-billion mass accuracy.
  • High accuracy necessitates careful consideration of mass calibration distortions.

Purpose of the Study:

  • To analytically model and numerically verify factors causing mass calibration distortions.
  • To improve the precision of mass measurements in time-of-flight mass spectrometry.

Main Methods:

  • Analytical modeling of calibration distortions.
  • Numerical verification of modeled effects.
  • Inclusion of relativistic corrections.

Main Results:

  • Quantified the influence of starting position on flight time.
  • Assessed the impact of accelerating field spatial gradients.
  • Evaluated distortions from accelerating pulse overshooting.
  • Confirmed the necessity of relativistic corrections for high accuracy.

Conclusions:

  • Precise mass calibration in TOF-MS demands control over identified distortion factors.
  • Analytical and numerical methods confirm the significance of these distortions.
  • Relativistic effects are crucial for achieving state-of-the-art mass accuracy.