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Ssn6 Interacts with Polar Tube Protein 2 and Transcriptional Repressor for RNA Polymerase II: Insight into Its

Runpeng Wang1, Yong Chen1, Sheng Xu1

  • 1College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China.

Journal of Fungi (Basel, Switzerland)
|October 27, 2023
PubMed

Insights

Nosema bombycis Ssn6 (Nbssn6) protein interacts with key components during silkworm infection. This interaction regulates the expression of polar tube and transcriptional repressor proteins, impacting parasite proliferation.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Parasitology

Background:

  • Nosema bombycis causes pebrine disease in silkworms, a significant economic issue in sericulture.
  • Microsporidia, including N. bombycis, utilize host cell machinery for infection and proliferation.
  • The general transcriptional corepressor Ssn6 is crucial in various cellular functions.

Purpose of the Study:

  • To isolate and characterize the Ssn6 gene (Nbssn6) from Nosema bombycis.
  • To investigate the cellular localization and protein interactions of Nbssn6.
  • To elucidate the role of Nbssn6 in the infection and proliferation of N. bombycis.

Main Methods:

  • Gene isolation and characterization of Nbssn6.
  • Indirect immunofluorescence assay for protein localization.
  • Co-immunoprecipitation (Co-IP) and yeast two-hybrid assays for protein interactions.
  • RNA interference (RNAi) for gene function analysis.

Main Results:

  • The Nbssn6 gene encodes a 393 amino acid protein, localized in the cytoplasm and nucleus during proliferation.
  • Nbssn6 interacts with Nbptp2 (polar tube protein 2) and Nbtrrp2 (transcriptional repressor for RNA polymerase II).
  • RNA interference confirmed that Nbssn6 regulates the transcription of Nbptp2 and Nbtrrp2.

Conclusions:

  • Nbssn6 plays a critical role in the infection and proliferation of Nosema bombycis.
  • Nbssn6 influences N. bombycis pathogenesis by interacting with polar tube proteins and transcriptional repressors.
  • Understanding Nbssn6 function provides insights into microsporidian host-parasite interactions.
Keywords:
general transcriptional corepressormicrosporidiapolar tube proteintranscriptional repressor for RNA polymerase II

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