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Dextranase Production Using Marine Microbacterium sp. XD05 and Its Application
Hind Boualis1,2, Xudong Wu1,2, Boyan Wang1,2
1Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China.
Marine Drugs
|October 27, 2023
Summary
A novel dextranase enzyme was isolated from Microbacterium sp. This enzyme efficiently hydrolyzes dextran into smaller oligosaccharides, showing potential for diverse industrial applications.
Area of Science:
- Enzymology
- Microbiology
Background:
- Dextranase (glucanase) is a hydrolase enzyme crucial for cleaving α-1,6 glycosidic bonds in dextran.
- Dextranase has potential applications across food, medicine, chemical, cosmetic, and agricultural industries.
Purpose of the Study:
- To isolate and characterize a novel dextranase-producing microbial strain.
- To optimize the conditions for dextranase production and evaluate its enzymatic properties.
Main Methods:
- Isolation and identification of a dextranase-producing strain from Qingdao Sea water samples (identified as Microbacterium sp. XD05).
- Optimization of growth and fermentation conditions (carbon/nitrogen sources, pH, temperature, time).
- Enzymatic characterization including thermal and pH stability, and analysis of hydrolysates using High-Performance Liquid Chromatography (HPLC).
Main Results:
- Microbacterium sp. XD05 was identified as an effective dextranase producer.
- Optimal fermentation occurred with sucrose and ammonium sodium at pH 7.5, 40 °C for 48 h.
- The enzyme exhibited good thermal stability (>70% activity at 40 °C) and stability in weak alkaline conditions (pH 6.0-8.0).
- Dextran hydrolysis yielded maltoheptaose, maltohexaose, maltopentaose, and malto-triose; corn porous starch formation was also observed.
Conclusions:
- A robust dextranase from Microbacterium sp. XD05 was characterized, demonstrating significant potential for industrial applications.
- The enzyme's stability and specific hydrolysis products make it a valuable candidate for various biotechnological processes.
