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A rapid assessment of percentage hemoglobin-S in whole blood
In an effort to bridge the gap between the rapid Sickledex screening test (Ortho Diagnostics, Raritan, NJ) and the slow but quantitative hemoglobin electrophoresis, the authors developed a rapid method for quantification of the percentage of hemoglobin S (%Hgb-S) in whole blood. The method involves quantification of the hemoglobin before and after removal of sodium dithionite-induced sickled cells for calculation of the %Hgb-S. Test results were within a +10% range of hemoglobin electrophoresis, with 67% of results within a +5% range. The method, taking 30-45 minutes to perform, offers a rapid assessment of %Hgb-S in acute care situations.
In an effort to bridge the gap between the rapid Sickledex screening test (Ortho Diagnostics, Raritan, NJ) and the slow but quantitative hemoglobin electrophoresis, the authors developed a rapid method for quantification of the percentage of hemoglobin S (%Hgb-S) in whole blood. The method involves quantification of the hemoglobin before and after removal of sodium dithionite-induced sickled cells for calculation of the %Hgb-S. Test results were within a +10% range of hemoglobin electrophoresis, with 67% of results within a +5% range. The method, taking 30-45 minutes to perform, offers a rapid assessment of %Hgb-S in acute care situations.