Jeotgalibaca caeni sp. nov., isolated from biochemical tank sludge

A Gram-stain-positive, coccoid-shaped, non-spore-forming, facultatively anaerobic bacterium, designated YN-L-12^T, was isolated from the activate sludge of a pesticide plant. Colonies on tryptone soya agar were small, white, opaque and circular. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YN-L-12^T belonged to the genus of Jeotgalibaca, and showed the highest similarity to Jeotgalibaca arthritidis 1805-02^T (97.0 %), followed by Jeotgalibaca ciconiae H21T32^T (96.5 %), Jeotgalibaca porci 1804-02^T (95.6 %) and Jeotgalibaca dankookensis EX-07^T (95.4 %). The strain grew at 15-37 °C (optimum, 30 °C), with 0-6.5 % (w/v) NaCl (optimum, 0.5 %) and at pH 7-9 (optimum, pH 7.5). The major fatty acids were C_18 : 1 ω9c, C_16 : 1 ω9c and C_16 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, glycolipid and an unidentified lipid. The DNA G+C content of the strain was 41.1 mol%. Average nucleotide identity values between strain YN-L-12^T and J. arthritidis 1805-02^T and J. ciconiae H21T32^T were 72.8 and 72.3 %, respectively. The digital DNA-DNA hybridization values between YN-L-12^T and J. arthritidis 1805-02^T and J. ciconiae H21T32^T were 24.1 and 20.3 %, respectively. According to the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain YN-L-12^T represents a novel species of the genus Jeotgalibaca, for which the name Jeotgalibaca caeni sp. nov. is proposed, with strain YN-L-12^T (=KCTC 43533^T=CCTCC AB 2022400^T) as the type strain.