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Structural insights on the nucleoprotein C-terminal domain of Měnglà virus

Diego Sebastian Ferrero1, Omar Tomás Gilabert1, Nuria Verdaguer1

  • 1Molecular Biology Institute of Barcelona (IBMB-CSIC) , Barcelona, Spain.

Microbiology Spectrum
|October 27, 2023
PubMed
Abstract

Insights

Měnglà virus (MLAV) nucleoprotein C-terminal domain (CTD) crystal structures reveal similarities to MARV. This MLAV NP CTD can form helical oligomers, potentially influencing viral replication.

Area of Science:

  • Virology
  • Structural Biology
  • Molecular Biology

Background:

  • Filoviruses cause severe hemorrhagic diseases.
  • Měnglà virus (MLAV), a bat-derived filovirus, replicates in human cells, posing a public health risk.
  • Understanding MLAV protein structure is crucial for developing antiviral strategies against emerging filoviruses.

Purpose of the Study:

  • To determine the crystal structures of the MLAV nucleoprotein C-terminal domain (NP CTD).
  • To investigate the structural similarities between MLAV NP CTD and other filoviruses.
  • To explore the oligomerization potential of the MLAV NP CTD and its role in viral replication.

Main Methods:

  • X-ray crystallography to determine the 3D structure of MLAV NP CTD.
  • Comparative structural analysis with known filovirus nucleoproteins.
  • Oligomerization assays to assess the self-assembly properties of MLAV NP CTD.

Main Results:

  • The first crystal structures of the MLAV NP CTD were determined.
  • MLAV NP CTD exhibits significant structural similarities to the equivalent domain in Marburg virus (MARV) NP.
  • MLAV NP CTD demonstrates the capacity to form large helical oligomers in vitro.

Conclusions:

  • The structural insights into MLAV NP CTD provide a foundation for antiviral drug development.
  • The oligomerization ability of MLAV NP CTD may be involved in the formation of cytoplasmic inclusion bodies during viral replication.
  • This study enhances our understanding of filovirus nucleoprotein structure-function relationships, applicable to other emerging filoviruses.
Keywords:
Měnglà virusfilovirusinclusion bodynucleoprotein

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