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Updated: Jul 12, 2025
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Diffuse Optical Spectroscopy for the Quantitative Assessment of Acute Ionizing Radiation Induced Skin Toxicity Using a Mouse Model
Published on: May 27, 2016
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Methods to assess radiation induced abscopal responses in mice
Eneko Garate-Soraluze1, Irantzu Serrano-Mendioroz1, María Rodriguez-Ruiz2
1Program of Immunology and Immunotherapy, Center for Applied Medical Research (CIMA), Pamplona, Spain.
Methods in Cell Biology
|October 27, 2023
Summary
Local immunotherapy combined with radiotherapy enhances anti-tumor immunity. This approach leverages radiation-induced cell death and T-lymphocyte priming for systemic immune responses against cancer.
Area of Science:
- Immunology
- Oncology
- Radiotherapy
Background:
- Radiotherapy (RT) can synergize with the immune system to eliminate cancer by inducing immunogenic cell death and enhancing tumor antigen presentation.
- Combining RT with immune checkpoint blockade (ICB) can elicit systemic anti-tumor immune responses, but current abscopal effects are insufficient for clinical application.
Purpose of the Study:
- To investigate the feasibility of a local immunotherapy treatment combined with external beam irradiation.
- To evaluate the immune-mediated anti-tumor effects of this combined approach in preclinical mouse models.
Main Methods:
- Utilized two asynchronous subcutaneous B16OVA tumors in syngeneic immunocompetent C57BL/6 mice.
- Administered local immunotherapy concurrently with external beam irradiation.
Main Results:
- The combined treatment demonstrated immune-mediated anti-tumor effects in mice.
- The described technique showed feasibility for intratumoral delivery and potential applicability to various mouse transplantable tumors.
Conclusions:
- Local immunotherapy combined with external beam irradiation is a feasible strategy to enhance anti-tumor immunity.
- This approach has the potential to improve systemic anti-tumor responses and can be adapted for diverse tumor types.
